@prefix : . @prefix cl: . @prefix go: . @prefix ro: . @prefix bfo: . @prefix dct: . @prefix iao: . @prefix obi: . @prefix owl: . @prefix rdf: . @prefix xml: . @prefix xsd: . @prefix af-c: . @prefix af-e: . @prefix af-m: . @prefix af-p: . @prefix af-q: . @prefix af-r: . @prefix af-x: . @prefix chmo: . @prefix pato: . @prefix rdfs: . @prefix skos: . @prefix af-cur: . @base . rdf:type owl:Ontology ; owl:versionIRI ; owl:imports , ; dct:issued "2026-07-08T12:00:00Z"^^xsd:dateTime ; dct:license ; dct:modified "2026-03-30T12:00:00Z"^^xsd:dateTime ; dct:rights , ; dct:title "AFO Fluorescence Domain Ontology (WD/2026/06)" ; owl:priorVersion ; owl:versionInfo "WD/2026/06" . ################################################################# # Annotation properties ################################################################# ### http://purl.allotrope.org/ontologies/property#AFX_0002796 af-x:AFX_0002796 rdf:type owl:AnnotationProperty . ### http://purl.allotrope.org/ontologies/property#AFX_0002798 af-x:AFX_0002798 rdf:type owl:AnnotationProperty . ### http://purl.allotrope.org/ontologies/property#AFX_0002809 af-x:AFX_0002809 rdf:type owl:AnnotationProperty . ### http://purl.org/dc/terms/source dct:source rdf:type owl:AnnotationProperty . ### http://www.w3.org/2004/02/skos/core#altLabel skos:altLabel rdf:type owl:AnnotationProperty . ### http://www.w3.org/2004/02/skos/core#changeNote skos:changeNote rdf:type owl:AnnotationProperty . ### http://www.w3.org/2004/02/skos/core#definition skos:definition rdf:type owl:AnnotationProperty . ### http://www.w3.org/2004/02/skos/core#example skos:example rdf:type owl:AnnotationProperty . ### http://www.w3.org/2004/02/skos/core#note skos:note rdf:type owl:AnnotationProperty . ### http://www.w3.org/2004/02/skos/core#prefLabel skos:prefLabel rdf:type owl:AnnotationProperty . ### http://www.w3.org/2004/02/skos/core#scopeNote skos:scopeNote rdf:type owl:AnnotationProperty . ################################################################# # Classes ################################################################# ### http://purl.allotrope.org/ontologies/process#AFP_0000185 af-p:AFP_0000185 rdf:type owl:Class ; rdfs:subClassOf af-p:AFP_0001782 ; skos:altLabel "FRET" , "fluorescence resonance energy transfer microscopy" , "fluorescent resonance energy transfer" ; skos:definition "A method for determining the distance between molecules using an energy transfer between two fluorescent chromophores (a donor and an acceptor) in close proximity (typically <10 nm) via a nonradiative, long-range dipole-dipole coupling mechanism. Energy transfer usually occurs between a cyan fluorescent protein (donor) and a yellow fluorescent protein (acceptor). [CHMO]" ; skos:prefLabel "fluorescence resonance energy transfer" . ### http://purl.allotrope.org/ontologies/process#AFP_0000211 af-p:AFP_0000211 rdf:type owl:Class ; rdfs:subClassOf af-p:AFP_0000851 ; skos:altLabel "scanning FCS" ; skos:definition "A type of spectroscopy where the spontaneous fluorescence intensity fluctuations of a sample are measured and correlated within a very small detection volume which is moving with respect to the sample. [CHMO]" ; skos:prefLabel "scanning fluorescence correlation spectroscopy" . ### http://purl.allotrope.org/ontologies/process#AFP_0000291 af-p:AFP_0000291 rdf:type owl:Class ; rdfs:subClassOf af-p:AFP_0001156 ; skos:altLabel "X-ray emission spectrometry" , "X-ray fluorescence spectrometry" , "X-ray fluorescence spectroscopy" , "XES" , "XRES" , "XRF" , "XRF spectrometry" , "XRF spectroscopy" . ### http://purl.allotrope.org/ontologies/process#AFP_0000309 af-p:AFP_0000309 rdf:type owl:Class ; rdfs:subClassOf af-p:AFP_0000291 ; skos:altLabel "WD-XRF" , "WDS" , "WDX" , "WDXRF" , "wavelength dispersive X-ray emission spectrometry" , "wavelength dispersive X-ray fluorescence spectrometry" , "wavelength dispersive X-ray fluorescence spectroscopy" , "wavelength dispersive X-ray spectrometry" , "wavelength dispersive X-ray spectroscopy" . ### http://purl.allotrope.org/ontologies/process#AFP_0000425 af-p:AFP_0000425 rdf:type owl:Class ; rdfs:subClassOf af-p:AFP_0001101 ; skos:altLabel "TXRF" , "total reflection X-ray fluorescence analysis" , "total reflection X-ray fluorescence spectrometry" . ### http://purl.allotrope.org/ontologies/process#AFP_0000577 af-p:AFP_0000577 rdf:type owl:Class ; rdfs:subClassOf af-p:AFP_0001749 ; skos:altLabel "fluorescence polarisation spectroscopy"@en-gb , "FA" , "fluorescence anisotropy measurement " , "fluorescence polarization spectroscopy" ; skos:definition "A method for measuring the binding interaction between two labelled molecules. The sample is excited with vertically polarized light and the polarization of the fluorescence is measured. [CHMO]" ; skos:prefLabel "fluorescence anisotropy" . ### http://purl.allotrope.org/ontologies/process#AFP_0000738 af-p:AFP_0000738 rdf:type owl:Class ; rdfs:subClassOf af-p:AFP_0002099 ; skos:altLabel "single molecule fluorescence spectrometry" , "single molecule fluorimetry" , "single molecule fluorometry" , "single molecule spectrofluorometry" , "single-molecule fluorescence spectrometry" , "single-molecule fluorimetry" , "single-molecule fluorometry" , "single-molecule spectrofluorometry" ; skos:definition "A type of spectroscopy where the fluorescence of a single molecule excited by ultraviolet light is detected as a function of wavelength. [CHMO]" ; skos:prefLabel "single-molecule fluorescence spectroscopy" . ### http://purl.allotrope.org/ontologies/process#AFP_0000851 af-p:AFP_0000851 rdf:type owl:Class ; rdfs:subClassOf af-p:AFP_0002099 ; skos:altLabel "FCS" , "fluroescence correlation spectrometry" ; skos:definition "A type of spectroscopy where the spontaneous fluorescence intensity fluctuations of a sample are measured and correlated within a very small detection volume. Ultraviolet light is used to stimulate fluorescence. [CHMO]" ; skos:prefLabel "fluorescence correlation spectroscopy" . ### http://purl.allotrope.org/ontologies/process#AFP_0000877 af-p:AFP_0000877 rdf:type owl:Class ; rdfs:subClassOf af-p:AFP_0002015 ; skos:altLabel "LIF spectrometry" , "LIF spectroscopy" , "laser induced fluorescence spectrometry" , "laser-induced fluorescence spectrometry" , "laser-induced fluorescence spectroscopy" . ### http://purl.allotrope.org/ontologies/process#AFP_0000890 af-p:AFP_0000890 rdf:type owl:Class ; rdfs:subClassOf af-p:AFP_0000211 ; skos:altLabel "RICS" , "raster-scanning image correlation spectroscopy" ; skos:definition "A type of spectroscopy where the spontaneous fluorescence intensity fluctuations of a sample are measured and correlated within a very small detection volume which is moving with respect to the sample. The temporal and spatial correlation between adjacent pixels, lines and frames is calculated allowing for the mapping of local translational diffusion coefficients. [CHMO]" ; skos:prefLabel "raster image correlation spectroscopy" . ### http://purl.allotrope.org/ontologies/process#AFP_0001115 af-p:AFP_0001115 rdf:type owl:Class ; rdfs:subClassOf af-p:AFP_0001556 ; skos:altLabel "ED-FRAP" , "ED/FRAP" , "enzyme dependent fluorescence recovery after photobleaching" ; skos:definition "A method for determining the distribution of enzyme activity in a sample by photobleaching a specific fluorescent metabolite during microscopy, and monitoring its enzymatically-driven recovery. [CHMO]" ; skos:prefLabel "enzyme-dependent fluorescence recovery after photobleaching" . ### http://purl.allotrope.org/ontologies/process#AFP_0001207 af-p:AFP_0001207 rdf:type owl:Class ; rdfs:subClassOf af-p:AFP_0001749 ; skos:definition "Any method that involves observing, or measuring the time take for, loss of fluorescence in a sample. [CHMO]" ; skos:prefLabel "fluorescence quenching method" . ### http://purl.allotrope.org/ontologies/process#AFP_0001209 af-p:AFP_0001209 rdf:type owl:Class ; rdfs:subClassOf af-p:AFP_0000291 ; skos:altLabel "SR-XES" , "SR-XRF " , "SXRF" , "synchrotron X-ray fluorescence" , "synchrotron radiation X-ray emission spectroscopy" , "synchrotron-based X-ray radiation fluorescence" . ### http://purl.allotrope.org/ontologies/process#AFP_0001214 af-p:AFP_0001214 rdf:type owl:Class ; rdfs:subClassOf af-p:AFP_0002099 ; skos:altLabel "GP spectroscopy" ; skos:definition "A type of spectroscopy where a spectral shift, in fluorescence spectrum, which depends on the phase of lipid membranes is measured. [CHMO]" ; skos:prefLabel "generalised polarisation spectroscopy"@en-gb , "generalized polarization spectroscopy" . ### http://purl.allotrope.org/ontologies/process#AFP_0001217 af-p:AFP_0001217 rdf:type owl:Class ; rdfs:subClassOf af-p:AFP_0002099 ; skos:altLabel "time resolved fluorescence spectroscopy" , "time resolved fluorimetry" , "time resolved fluorometry" , "time resolved spectrofluorometry" , "time-resolved fluorimetry" , "time-resolved fluorometry" , "time-resolved spectrofluorometry" ; skos:definition "A type of spectroscopy where the time decay of the fluorescence of molecules excited using ultraviolet light is detected. [CHMO]" ; skos:prefLabel "time-resolved fluorescence spectroscopy" . ### http://purl.allotrope.org/ontologies/process#AFP_0001322 af-p:AFP_0001322 rdf:type owl:Class ; rdfs:subClassOf af-p:AFP_0002432 ; skos:altLabel "fluorescence imaging" . ### http://purl.allotrope.org/ontologies/process#AFP_0001369 af-p:AFP_0001369 rdf:type owl:Class ; rdfs:subClassOf af-p:AFP_0000851 ; skos:altLabel "FCCS" , "fluorescence cross-correlation spectrometry" ; skos:definition "A type of spectroscopy where spontaneous fluorescence intensity fluctuations of a sample are measured at two distinct wavelengths and correlated within a very small detection volume. Ultra-violet light is used to stimulate fluorescence. [CHMO]" ; skos:prefLabel "fluorescence cross-correlation spectroscopy" . ### http://purl.allotrope.org/ontologies/process#AFP_0001388 af-p:AFP_0001388 rdf:type owl:Class ; rdfs:subClassOf af-p:AFP_0001782 ; skos:altLabel "BRET" ; skos:definition "A method for determining the distance between molecules using an energy transfer between two chromophores (a donor and an acceptor) in close proximity (typically <10 nm) via a nonradiative, long-range dipole-dipole coupling mechanism. Energy transfer usually occurs between a luciferase (donor) and a green fluorescent protein (acceptor). The luciferase is bioluminescent therefore external illumination is not required. [CHMO]" ; skos:prefLabel "bioluminescence resonance energy transfer" . ### http://purl.allotrope.org/ontologies/process#AFP_0001411 af-p:AFP_0001411 rdf:type owl:Class ; rdfs:subClassOf af-p:AFP_0002099 ; skos:altLabel "EEM" , "excitation emission matrix (EEM) fluorescence" , "excitation-emission matrix fluorescence" , "excitation-emission matrix fluorescence spectroscopy" , "fluorescence EEM" ; skos:definition "A type of fluorescence spectroscopy where a two-dimensional total fluorescence spectrum is recorded at various excitationnwavelengths. [CHMO]" ; skos:prefLabel "excitation-emission matrix fluorescence spectroscopy" . ### http://purl.allotrope.org/ontologies/process#AFP_0001448 af-p:AFP_0001448 rdf:type owl:Class ; rdfs:subClassOf af-p:AFP_0002099 ; skos:altLabel "DSF" , "differential scanning fluorimetry" ; skos:definition "A type of spectroscopy where the intensity of the fluorescence emitted by molecules excited using ultraviolet light is detected as it changes upon interaction of the molecule with its environment. [CHMO]" ; skos:prefLabel "differential scanning fluorescence spectroscopy" . ### http://purl.allotrope.org/ontologies/process#AFP_0001467 af-p:AFP_0001467 rdf:type owl:Class ; rdfs:subClassOf af-p:AFP_0000577 ; skos:altLabel "time-resolved fluorescence polarisation spectroscopy"@en-gb , "TRFA" , "time resolved FA" , "time resolved fluorescence anisotropy" , "time-resolved FA" , "time-resolved fluorescence polarization spectroscopy" ; skos:definition "A method for measuring the binding interaction between two labelled molecules. The sample is excited with vertically polarized light and the decay time and polarization of the fluorescence is measured. [CHMO]" ; skos:prefLabel "time-resolved fluorescence anisotropy" . ### http://purl.allotrope.org/ontologies/process#AFP_0001556 af-p:AFP_0001556 rdf:type owl:Class ; rdfs:subClassOf af-p:AFP_0001749 ; skos:altLabel "FRAP" ; skos:definition "A method for determining the mobility of fluorescent molecules by photobleaching a sub-set of the sample during fluorescence microscopy and monitoring the movement of the remaining fluorescent molecules. [CHMO]" ; skos:prefLabel "fluorescence recovery after photobleaching" . ### http://purl.allotrope.org/ontologies/process#AFP_0001782 af-p:AFP_0001782 rdf:type owl:Class . ### http://purl.allotrope.org/ontologies/process#AFP_0001820 af-p:AFP_0001820 rdf:type owl:Class ; rdfs:subClassOf af-p:AFP_0001214 ; skos:altLabel "Laurdan generalized polarisation spectroscopy"@en-gb , "generalized polarisation of Laurdan fluorescence"@en-gb , "laurdan generalized polarisation (GP) measurements"@en-gb , "Laurdan generalized polarization spectroscopy" , "generalized polarization of Laurdan fluorescence" , "laurdan fluorescence spectroscopy" , "laurdan generalized polarization (GP) measurements" ; skos:definition "A type of spectroscopy where the difference between the red and blue fluorescence emission of the fluorescent probe laurdan (6-dodecanoyl-2- dimethylaminonaphthalene), which depends on the phase of lipid membranes, is measured. [CHMO]" ; skos:prefLabel "laurdan generalised polarisation spectroscopy"@en-gb , "laurdan generalized polarization spectroscopy" . ### http://purl.allotrope.org/ontologies/process#AFP_0001894 af-p:AFP_0001894 rdf:type owl:Class ; rdfs:subClassOf af-p:AFP_0000851 ; skos:altLabel "SA-FCS" , "supercritical angle FCS" ; skos:definition "A type of spectroscopy where the spontaneous fluorescence intensity fluctuations of a sample emitted at supercritical angles are measured and correlated within a very small detection volume. Ultra-violet light is used to stimulate fluorescence. [CHMO]" ; skos:prefLabel "supercritical angle fluorescence correlation spectroscopy" . ### http://purl.allotrope.org/ontologies/process#AFP_0001929 af-p:AFP_0001929 rdf:type owl:Class ; rdfs:subClassOf af-p:AFP_0000851 ; skos:altLabel "high pressure FCS" , "high pressure fluorescence correlation spectroscopy" , "high-pressure FCS" ; skos:definition "A type of spectroscopy where the spontaneous fluorescence intensity fluctuations of a sample are measured and correlated within a very small detection volume. Ultraviolet light is used to stimulate fluorescence and the sample is held inside a single fused silica microcapillary under high pressure (up to 300 MPa). [CHMO]" ; skos:prefLabel "high-pressure fluorescence correlation spectroscopy" . ### http://purl.allotrope.org/ontologies/process#AFP_0001930 af-p:AFP_0001930 rdf:type owl:Class ; rdfs:subClassOf af-p:AFP_0001280 ; skos:altLabel "USX" , "USX spectrometry" , "USX spectroscopy" , "USXES" , "USXS" , "ultra soft X-ray emission spectrometry" , "ultra soft X-ray fluorescence spectrometry" , "ultra soft X-ray fluorescence spectroscopy" , "ultra-soft X-ray emission spectrometry" , "ultra-soft X-ray fluorescence spectrometry" , "ultra-soft X-ray fluorescence spectroscopy" . ### http://purl.allotrope.org/ontologies/process#AFP_0002117 af-p:AFP_0002117 rdf:type owl:Class ; rdfs:subClassOf af-p:AFP_0002099 ; skos:altLabel "steady state fluorescence spectroscopy" , "steady state fluorimetry" , "steady state fluorometry" , "steady state spectrofluorometry" , "steady-state fluorimetry" , "steady-state fluorometry" , "steady-state spectrofluorometry" ; skos:definition "A type of spectroscopy where the intensity of the fluorescence emitted by molecules excited by constant illumination of ultraviolet light is detected as a function of wavelength. [CHMO]" ; skos:prefLabel "steady-state fluorescence spectroscopy" . ### http://purl.allotrope.org/ontologies/process#AFP_0002144 af-p:AFP_0002144 rdf:type owl:Class ; rdfs:subClassOf af-p:AFP_0000851 ; skos:altLabel "TIR-FCS" , "total internal reflection FCS" ; skos:definition "A type of spectroscopy where ultraviolet light is used to stimulate fluorescence in a sample, and the spontaneous fluorescence intensity fluctuations are measured and correlated within a very small detection volume. To generate total internal reflection the UV light is either passed through a prism to generate high enough incident angles, or the UV light is focused through the periphery of a high numerical aperture objective which is directly coupled to the sample surface. [CHMO]" ; skos:prefLabel "total internal reflection fluorescence correlation spectroscopy" . ### http://purl.allotrope.org/ontologies/process#AFP_0002164 af-p:AFP_0002164 rdf:type owl:Class ; rdfs:subClassOf af-p:AFP_0001207 ; skos:altLabel "TRFQ" , "TRFQ analysis" , "TRFQ measurements" , "TRFQ spectrometry" , "TRFQ spectroscopy" , "time resolved fluorescence quenching" , "time resolved fluorescence quenching spectrometry" , "time-resolved fluorescence quenching spectrometry" , "time-resolved fluorescence quenching spectroscopy" ; skos:definition "The measurement of the fluorescence decay time of probes solubilised in micellar surfactant solutions in the presence of quenchers. [CHMO]" ; skos:prefLabel "time-resolved fluorescence quenching" . ### http://purl.allotrope.org/ontologies/process#AFP_0002241 af-p:AFP_0002241 rdf:type owl:Class ; rdfs:subClassOf af-p:AFP_0000851 ; skos:altLabel "confocal FCS" ; skos:definition "A type of spectroscopy where the spontaneous fluorescence intensity fluctuations of a sample are measured and correlated within a very small detection volume. The light emitted during fluorescence is collected and guided through a pinhole before it is detected, reducing background interference. [CHMO]" ; skos:prefLabel "confocal fluorescence correlation spectroscopy" . ### http://purl.allotrope.org/ontologies/process#AFP_0002312 af-p:AFP_0002312 rdf:type owl:Class ; rdfs:subClassOf af-p:AFP_0000851 ; skos:altLabel "ICS" ; skos:definition "A type of spectroscopy where the spontaneous fluorescence intensity fluctuations of a sample are measured at several different points in parallel and correlated within very small detection volumes. Ultra-violet light is used to stimulate fluorescence. [CHMO]" ; skos:prefLabel "image correlation spectroscopy" . ### http://purl.allotrope.org/ontologies/result#AFR_0000109 af-r:AFR_0000109 rdf:type owl:Class ; rdfs:subClassOf af-r:AFR_0000136 , af-r:AFR_0000357 ; skos:definition "A fluorescence spectrum where the excitation process involves two-photon absorption. [CHMO]" ; skos:prefLabel "two-photon excited fluorescence spectrum" . ### http://purl.allotrope.org/ontologies/result#AFR_0000115 af-r:AFR_0000115 rdf:type owl:Class ; rdfs:subClassOf af-r:AFR_0000390 ; skos:altLabel "fluorescence anisotropy decay curves" , "fluorescence anisotropy decays" ; skos:definition "A plot of fluorescence anisotropy (the difference in polarisation between incident and fluorescent light) vs. time. [CHMO]" ; skos:prefLabel "fluorescence anisotropy decay curve" . ### http://purl.allotrope.org/ontologies/result#AFR_0000156 af-r:AFR_0000156 rdf:type owl:Class ; rdfs:subClassOf af-r:AFR_0000329 ; skos:definition "A fluorescence image obtained through a two-photon excitation process. [CHMO]" ; skos:prefLabel "two-photon fluorescence image" . ### http://purl.allotrope.org/ontologies/result#AFR_0000329 af-r:AFR_0000329 rdf:type owl:Class ; rdfs:subClassOf iao:_0000101 ; skos:definition "A 2D image obtained by measuring fluorescence. [CHMO]" ; skos:prefLabel "fluorescence image" . ### http://purl.allotrope.org/ontologies/result#AFR_0000376 af-r:AFR_0000376 rdf:type owl:Class ; rdfs:subClassOf af-r:AFR_0000136 , af-r:AFR_0000412 ; skos:altLabel "excitation-emission fluorescence spectra" , "excitation-emission fluorescence spectrum" ; skos:definition "A 3D plot of excitation wavelength vs. emission wavelength vs. intensity obtained from a fluorescence spectroscopy experiment. [CHMO]" ; skos:prefLabel "excitation-emission fluorescence spectrum" . ### http://purl.allotrope.org/ontologies/result#AFR_0000390 af-r:AFR_0000390 rdf:type owl:Class ; rdfs:subClassOf af-r:AFR_0000068 ; skos:altLabel "fluorescence decay curves" ; skos:definition "A plot of fluorescence intensity vs. time. [CHMO]" ; skos:prefLabel "fluorescence decay curve" . ################################################################# # Individuals ################################################################# ### http://purl.allotrope.org/voc/attribution rdf:type owl:NamedIndividual , dct:RightsStatement ; dct:description """ These taxonomies contain material or may constitute derivative works of material which may be subject to copyright by one of the following organizations. 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Copyright © 2015-2026 Allotrope Foundation """ ; dct:title "Creative Commons Attribution 4.0 International Public License" . ### Generated by the OWL API (version 5.5.1) https://github.com/owlcs/owlapi/