AFO Chromatography Domain Ontology (REC/2026/06)
type: Ontology
imports: afo
imports: detection
imports: injection
imports: sampling
contributor: Allotrope Foundation
issued: 2026-07-08T12:00:00Z
license: Creative Commons Attribution 4.0 International Public License
license: iupac-license
modified: 2026-07-07T12:00:00Z
publisher: Allotrope Foundation
rights: Derivative works attribution
rights: Copyright © 2015-2026 Allotrope Foundation
rights holder: Allotrope Foundation
prior version: chromatography
version info: REC/2026/06

capillary column
type: Class
subclass of: AFE_0000217
AFX_0002798: true
AFX_0002809: CHMO_0000987
definition: A piece of apparatus that has the form of a tube with a diameter of less than a millimeter and hosts the stationary bed in chromatography. [CHMO]

porous-layer open-tubular column
type: Class
subclass of: open-tubular column
AFX_0002798: true
AFX_0002808: CHMO_0000972
alternative label: PLOT column
definition: An open-tubular column in which a porous layer, achieved by either etching a non-porous layer or by deposition of porous particles from a suspension, coats the inner wall and may serve as a support for a liquid stationary phase or as the stationary phase itself. [CHMO]

analytical column
type: Class
subclass of: AFE_0000217
AFX_0002798: true
definition: A chromatography column used for analysis. [Allotrope]

open chromatographic bed
type: Class
subclass of: AFE_0000354
definition: A piece of apparatus that is planar and is or hosts the stationary bed in chromatography. [CHMO]

chromatographic detector
type: Class
subclass of: Class
  intersection of: AFE_0000317
  intersection of: Restriction
    on property: RO_0000056
    some values from: AFP_0001321
AFX_0002798: true
AFX_0002808: OBI_0000481
source: PAC, 1993, 65, 819 (Nomenclature for chromatography (IUPAC Recommendations 1993))
definition: A chromatography detector is a detector that is used in chromatography as component of a chromatography system. [Allotrope]

strong anion-exchange column
type: Class
subclass of: anion-exchange column
AFX_0002798: true
AFX_0002808: CHMO_0002268
alternative label: SAX column
definition: A chromatography column where the stationary phase is a strong anion exchanger. [CHMO]

weak anion-exchange column
type: Class
subclass of: anion-exchange column
AFX_0002798: true
AFX_0002808: CHMO_0002269
alternative label: WAX column
definition: A chromatography column where the stationary phase is a weak anion exchanger. [CHMO]

support-coated open-tubular column
type: Class
subclass of: porous-layer open-tubular column
AFX_0002798: true
AFX_0002808: CHMO_0000971
alternative label: SCOT column
definition: An open-tubular column in which a layer of porous particles deposited from a suspension coats the inner wall and serves as a support for a liquid stationary phase. [CHMO]

strong cation-exchange column
type: Class
subclass of: cation-exchange column
AFX_0002798: true
AFX_0002808: CHMO_0002266
alternative label: SCX column
definition: A chromatography column where the stationary phase is a strong cation exchanger. [CHMO]

guard column
type: Class
subclass of: AFE_0000217
AFX_0002798: true
AFX_0002808: OBI_0000614
definition: A short protective chromatography column used to protect the main analytical column from damaging impurities that are injected into the instrument. [Allotrope]

gel filtration chromatograph
type: Class
subclass of: AFE_0000392
AFX_0002798: true
alternative label: GPC
alternative label: gel permeation chromatograph
definition: A piece of apparatus, consisting of an eluent supply system, an injection or sampling system, a column containing a swollen gel, a detector and a data acquisition/processing system, that is used to carry out chromatographic separations. [CHMO]

cation-exchange column
type: Class
subclass of: ion-exchange column
AFX_0002798: true
AFX_0002808: CHMO_0002263
definition: A chromatography column where the stationary phase is a cation exchanger. [CHMO]

anion-exchange column
type: Class
subclass of: ion-exchange column
AFX_0002798: true
AFX_0002808: CHMO_0002265
definition: A chromatography column where the stationary phase is an anion exchanger. [CHMO]

capillary electrophoresis column
type: Class
subclass of: AFE_0000217
AFX_0002798: true
definition: A capillary electrophoresis column is a type of column that is used in capillary electrophoresis. [Allotrope]

open-tubular column
type: Class
subclass of: AFE_0000217
AFX_0002798: true
AFX_0002808: OBI_0000606
definition: A chromatography column in which either the inner tube wall or a liquid or active solid held stationary on the tube wall acts as the stationary phase and there is an open, unrestricted path for the mobile phase. [CHMO]

packed column
type: Class
subclass of: AFE_0000217
AFX_0002798: true
AFX_0002808: CHMO_0000975
definition: A chromatography column that contains a solid packing. [CHMO]

array column
type: Class
subclass of: AFE_0000217
AFX_0002798: true
definition: An array column is a column that contains multiple columns at the same time. [Allotrope]

weak cation-exchange column
type: Class
subclass of: cation-exchange column
AFX_0002798: true
AFX_0002808: CHMO_0002267
alternative label: WCX column
definition: A chromatography column where the stationary phase is a weak cation exchanger. [CHMO]

wall-coated open-tubular column
type: Class
subclass of: open-tubular column
AFX_0002798: true
AFX_0002808: CHMO_0000973
alternative label: WCOT column
definition: An open-tubular column in which the liquid stationary phase is coated on the unmodified smooth inner wall of the tube. [CHMO]

ion-exchange column
type: Class
subclass of: AFE_0000217
AFX_0002798: true
AFX_0002808: CHMO_0002264
definition: A chromatography column where the stationary phase is an ion exchanger. [CHMO]

spin column
type: Class
subclass of: AFE_0000217
AFX_0002798: true
AFX_0002808: OBI_0000570
alternative label: spin concentrator
definition: A spin column is a column used for concentration of biological samples containing membranes for ultrafiltration. The solid phase is made up of silica to which the biological sample binds. [Allotrope]

bypass injector
type: Class
subclass of: AFE_0000266
AFX_0002798: true
license: iupac-license
rights: iupac-copyright
source: L.S. Ettre, "Nomenclature for Chromatography", Pure & Appl. Chem. 1993, Vol. 65, No. 4, pp. 819-872
alternative label: sampling valve
alternative label: valve injector
definition: A device in which the sample is first introduced into a chamber (loop), temporarily isolated from the mobile phase system by valves, which can be switched to make an instantaneous diversion of the mobile phase stream through the chamber to carry the sample to the column. [IUPAC]

direct injector
type: Class
subclass of: AFE_0000266
AFX_0002798: true
license: iupac-license
rights: iupac-copyright
source: L.S. Ettre, "Nomenclature for Chromatography", Pure & Appl. Chem. 1993, Vol. 65, No. 4, pp. 819-872
definition: A device which directly introduces the sample into the mobile-phase stream. [IUPAC]

on-column injector
type: Class
subclass of: AFE_0000266
AFX_0002798: true
license: iupac-license
rights: iupac-copyright
source: L.S. Ettre, "Nomenclature for Chromatography", Pure & Appl. Chem. 1993, Vol. 65, No. 4, pp. 819-872
definition: A device which directly introduces the sample into the mobile-phase stream. [IUPAC]

column compartment
type: Class
subclass of: AFE_0000322
definition: A column compartment is a compartment in a chromatography system where the columns are held. [Allotrope]
editorial note: 2019-04-01 Adding 'ro:location of' some 'af-e:chromatography column' breaks Hermit reasoner. [Allotrope]

monolithic column
type: Class
subclass of: AFE_0000217
definition: A monolithic columns is a chromatography column where the stationary phase synthesized in place as a single lattice structure in the column. [Allotrope]

chromatography data system
type: Class
subclass of: AFE_0002232
alternative label: CDS
definition: A chromatography data system is a data system that controls a chromatography system and processes data participating in a chromatography run. [Allotrope]

to deliver material
type: Class
equivalent class: Class
  intersection of: AFFN_0000113
  intersection of: Restriction
    on property: BFO_0000054
    some values from: AFP_0003575
subclass of: AFFN_0000113
definition: To deliver material is the function to transfer a material to or into a destination. [Allotrope]

to deliver mobile phase
type: Class
equivalent class: Class
  intersection of: to deliver material
  intersection of: Restriction
    on property: BFO_0000054
    some values from: Class
      intersection of: AFP_0003349
      intersection of: Restriction
        on property: AFX_0002745
        some values from: mobile phase
subclass of: to deliver material
AFX_0002798: true
definition: To deliver mobile phase is the function to deliver material that is in a mobile phase role. [Allotrope]

to separate material by chromatography
type: Class
equivalent class: Class
  intersection of: AFFN_0000031
  intersection of: AFFN_0000107
  intersection of: Restriction
    on property: BFO_0000054
    some values from: AFP_0001321
subclass of: AFFN_0000105
AFX_0002798: true
alternative label: separation by chromatography function
definition: A material separation function which is realized by chromatography. [Allotrope]

chromatographic packing
type: Class
subclass of: AFM_0001042
definition: A chromatographic packing is a solid heterogeneous mixture consisting of a substrate and stationary phase used in a chromatographic separation. [Allotrope]

partition chromatography
type: Class
subclass of: AFP_0001321
definition: Chromatography in which separation is based mainly on differences between the solubility of the sample components in the stationary phase (gas chromatography), or on differences between the solubilities of the components in the mobile and stationary phases (liquid chromatography). [CHMO]

column chromatography
type: Class
equivalent class: Class
  intersection of: AFP_0001321
  intersection of: Restriction
    on property: RO_0000057
    some values from: AFE_0000217
subclass of: AFP_0001321
AFX_0002798: true
definition: A chromatography method where the stationary bed is within a tube (of standard length 25 cm). The particles of the solid stationary phase or support coated with a liquid stationary phase may fill the whole inside volume of the tube (packed column) or be concentrated on or along the inside tube wall leaving an open, unrestricted path for the mobile phase in the middle part of the tube (open-tubular column). [CHMO]

adsorption chromatography
type: Class
subclass of: AFP_0001321
definition: Chromatography in which separation is based mainly on differences between the adsorption affinities of the sample components for the surface of an active solid. [CHMO]

normal-phase chromatography
type: Class
subclass of: AFP_0001321
AFX_0002798: true
alternative label: NPC
alternative label: normal phase chromatography
definition: A separation method where the components are distributed between two phases, one of which is stationary and polar, while the other is non-polar and moves in a definite direction. [CHMO]

injection (chromatography)
type: Class
subclass of: Class
  intersection of: AFP_0003666
  intersection of: AFP_0003813
AFX_0002818: true
alternative label: injection
alternative label: sample introduction
change note: 2018-10-24 Moved to chromatography domain ontology, subclassed under loading [Allotrope]
change note: 2022-06-20 Subclassed under sample introduction [Allotrope]
definition: Process of loading a sample into a chromatography system. [Allotrope]

chiral chromatography
type: Class
subclass of: AFP_0001321
AFX_0002798: true
definition: A separation method where the components (in this case stereoisomers) are distributed between two chiral phases, one of which is stationary, while the other moves in a definite direction. [CHMO]

planar chromatography
type: Class
subclass of: AFP_0001321
alternative label: open bed chromatography
alternative label: open-bed chromatography
definition: A chromatography method where the stationary phase is present as or on a plane. [CHMO]

chromatography data processing
type: Class
subclass of: AFP_0003598
change note: 2019-09-09 Moved under data transformation and fixed definition. [Allotrope]
definition: Chromatography data processing is a data transformation on chromatograms. [Allotrope]

displacement chromatography
type: Class
subclass of: AFP_0001321
definition: Chromatography where the mobile phase contains a compound (the displacer) more strongly retained than the components of the sample under examination. The sample is fed into the system as a finite slug. [CHMO]

reversed-phase chromatography
type: Class
subclass of: AFP_0001321
AFX_0002798: true
alternative label: RPC
alternative label: reverse phase chromatography
alternative label: reverse-phase chromatography
alternative label: reversed phase chromatography
definition: A separation method where the components are distributed between two phases, one of which is stationary and non-polar, while the other is polar and moves in a definite direction. [CHMO]

headspace chromatography
type: Class
subclass of: AFP_0001321
definition: Headspace chromatography is a type of chromatography that uses the gaseous headspace above a sample for analysis because very light volatiles can be efficiently partitioned as they diffuse to the gas volume above. [Allotrope]

mixed-mode chromatography
type: Class
subclass of: AFP_0001321
alternative label: multimodal chromatography
definition: Mixed-mode chromatography (MMC), or multimodal chromatography, refers to chromatographic methods that utilize more than one form of interaction between the stationary phase and analytes in order to achieve separation. [Wikipedia]

membrane chromatography
type: Class
subclass of: AFP_0001321
definition: A chromatography method where the separation is based on the adsorption to membranes. [Allotrope]

mobile phase delivery
type: Class
subclass of: Class
  intersection of: BFO_0000015
  intersection of: Restriction
    on property: BFO_0000051
    some values from: AFP_0001135
  intersection of: Restriction
    on property: BFO_0000051
    some values from: AFP_0003312
change note: 2019-09-20 Changed definition. [Allotrope]
definition: Mobile phase delivery is a process performed by a chromatography system in which the mobile phase is mixed and transported through the instrument for separation and detection. [Allotrope]

relative retention calculation
type: Class
subclass of: AFP_0003282
definition: The relative retention calculation is a process of performing the mathematical ratio quantification of the time a substance and a standard that remain in the stationary phase of a chromatography process. [Allotrope]

constant pressure mobile phase delivery
type: Class
subclass of: mobile phase delivery
definition: Constant pressure mobile phase delivery is a mobile phase delivery process whereby the mobile phase is delivered by maintaining a constant pressure of the mobile phase. [Allotrope]

constant flow rate mobile phase delivery
type: Class
subclass of: mobile phase delivery
definition: Constant flow rate mobile phase delivery is a mobile phase delivery process whereby the mobile phase is delivered by maintaining a constant flow rate of the mobile phase. [Allotrope]

chromatography column length (quality)
type: Class
equivalent class: Class
  intersection of: AFQ_0000020
  intersection of: Restriction
    on property: RO_0000080
    some values from: AFE_0000217
AFX_0002798: true
AFX_0002811: AFX_0000904
alternative label: chromatography column length
change note: 2020-06-22 Changed labels. [Allotrope]
definition: The length of a chromatography column bed. [Allotrope]

column inner diameter (quality)
type: Class
equivalent class: Class
  intersection of: AFQ_0000018
  intersection of: Restriction
    on property: RO_0000080
    some values from: AFE_0000217
AFX_0002798: true
alternative label: column inner diameter
alternative label: dc
alternative label: inner diameter
change note: 2020-06-22 Changed labels and definition. [Allotrope]
definition: Column inner diameter is the inner diameter of a column. [Allotrope]

AFQ_0000018
type: Class
alternative label: inner diameter
change note: 2020-06-22 Changed labels. [Allotrope]

relative retention time (quality)
type: Class
subclass of: AFQ_0000005
AFX_0002811: AFX_0001180
AFX_0002818: true
source: Orange Book: IUPAC Compendium of Analytical Nomenclature. Second Edition, Blackwell Scientific Publications, Oxford, 1987.
source: PAC, 1993, 65, 819. Nomenclature for chromatography (IUPAC Recommendations 1993)
alternative label: relative retention
alternative label: relative retention time
definition: Relative retention time is a ratio quality of the adjusted or net retention time of a component relative to that of a standard, obtained under identical conditions. [Allotrope]

hold-up volume (quality)
type: Class
subclass of: PATO_0000918
license: iupac-license
rights: iupac-copyright
source: PAC, 1993, 65, 819 (Nomenclature for chromatography (IUPAC Recommendations 1993))
alternative label: dead volume
alternative label: hold-up volume
definition: The hold-up volume is the volume of the mobile phase required to elute a component the concentration of which in the stationary phase is negligible compared to that in the mobile phase. In other words, this component is not retained at all by the stationary phase. Thus, the hold-up volume is equal to the retention volume of an unretained compound. [IUPAC]

retention volume (quality)
type: Class
subclass of: PATO_0000918
license: iupac-license
rights: iupac-copyright
source: PAC, 1993, 65, 819 (Nomenclature for chromatography (IUPAC Recommendations 1993))
alternative label: retention volume
definition: Retention volume is the volume of the mobile phase required to elute a component the concentration of which in the stationary phase is negligible compared to that in the mobile phase. In other words, this component is not retained at all by the stationary phase. Thus, the hold-up volume is equal to the retention volume of an unretained compound. The hold-up volume includes any volumes contributed by the sample injector, the detector, and connectors. In gas chromatography this term is also called the gas hold-up volume. The corrected gas hold-up volume is the gas hold-up volume multiplied by the compression (compressibility) correction factor. [IUPAC]

bonded phase
type: Class
subclass of: PATO_0000141
definition: A bonded phase is the structure quality of a material that is covalently bonded to a substrate. [Allotrope]

chromatography peak
type: Class
subclass of: AFR_0000413
definition: A chromatography peak is a peak that results from a detector's response to the presence of an analyte in the mobile phase eluting from a column in chromatography. [Allotrope]

gas chromatogram
type: Class
subclass of: AFR_0000236
alternative label: GC chromatogram
definition: A plot of detector signal (e.g. ion count or absorbance) vs. retention time obtained from a column chromatography experiment where the mobile phase is a gas. [CHMO]

mass chromatogram
type: Class
subclass of: AFR_0000236
definition: A mass chromatogram is a distribution function of a mass detector signal versus time of acquisition or versus volume of analyte. [Allotrope]

electromagnetic radiation chromatogram
type: Class
subclass of: AFR_0000236
alternative label: EMR chromatogram
definition: An electromagnetic radiation chromatogram is a distribution function of a electromagnetic radiation detector signal versus time of acquisition or versus volume of analyte. [Allotrope]

size-exclusion chromatogram
type: Class
subclass of: AFR_0000236
alternative label: SEC chromatogram
alternative label: SEC elugram
alternative label: SEC profile
alternative label: SEC trace
alternative label: size exclusion chromatogram
definition: A plot of detector signal (e.g. ion count or absorbance) vs. retention time obtained from a chromatography experiment where the separation is caused by exclusion effects within the stationary phase and where the stationary phase is a swollen gel. [CHMO]

gel filtration chromatogram
type: Class
subclass of: AFR_0000236
alternative label: GPC elugram
alternative label: GPC profile
alternative label: GPC profiles
alternative label: GPC trace
alternative label: GPC traces
alternative label: gel permeation chromatogram
alternative label: gel permeation chromatographic traces
definition: A plot of detector signal (e.g. ion count or absorbance) vs. retention time obtained from a chromatography experiment where the separation is caused by differences in molecular size. [CHMO]

unretained peak
type: Class
subclass of: AFR_0000413
AFX_0002798: true
definition: An unretained peak is a peak which represents the detection result of a material entity which was not retained by a chromatography column. [Allotrope]

retention time
type: Class
subclass of: AFR_0001101
subclass of: chromatography peak facet
change note: 2020-03-20 Added quantification facet superclass. [Allotrope]
definition: Retention time is a chromatography peak facet that indicates the elapsed time between the injection of the sample and the maximum detector response attributed to the peak (analyte band). [Allotrope]

relative retention time
type: Class
subclass of: AFR_0001101
subclass of: Class
  intersection of: chromatography peak facet
  intersection of: Restriction
    on property: AFX_0002730
    some values from: AFRL_0000248
  intersection of: Restriction
    on property: AFX_0002816
    some values from: relative retention time (quality)
change note: 2020-03-20 Added quantification facet superclass. [Allotrope]
definition: A chromatography peak facet that denotes the retention time of the peak relative to some other specified reference peak. [Allotrope]

unadjusted relative retention
type: Class
subclass of: Class
  intersection of: relative retention time
  intersection of: Restriction
    on property: AFX_0002730
    some values from: AFRL_0000248
definition: A relative retention time that quantitates the ratio of the retention time of the peak relative to some other specified reference peak. [Allotrope]

adjusted relative retention
type: Class
subclass of: Class
  intersection of: relative retention time
  intersection of: Restriction
    on property: AFX_0002730
    some values from: AFRL_0000248
definition: A relative retention time that quantitates the ratio of the adjusted retention time (corrected for hold-up time) of the peak relative to the adjusted retention time of some reference peak. [Allotrope]

chromatography peak facet
type: Class
equivalent class: Class
  intersection of: AFR_0000947
  intersection of: Restriction
    on property: AFX_0002800
    some values from: chromatography peak
AFX_0002818: true
definition: A chromatography peak facet is a facet of a chromatography peak. [Allotrope]

AFR_0001197
change note: 2019-09-26 Removed alt labels, now their own subclasses. [Allotrope]

baseline (chromatography)
type: Class
subclass of: AFR_0001219
license: iupac-license
rights: iupac-copyright
source: PAC, 1990, 62, 2167 (Glossary of atmospheric chemistry terms (Recommendations 1990))
source: PAC, 1993, 65, 819 (Nomenclature for chromatography (IUPAC Recommendations 1993))
alternative label: baseline
definition: The portion of the chromatogram recording the detector response when only the mobile phase emerges from the column. [IUPAC]

chromatographic peak resolution
type: Class
subclass of: Class
  intersection of: chromatography peak facet
  intersection of: AFR_0001199
definition: A chromatographic peak facet that quantitates the degree of separation between two peaks of a chromatogram. [Allotrope]

chromatographic peak resolution using baseline peak widths
type: Class
subclass of: chromatographic peak resolution
alternative label: chromatographic peak resolution by USP <621> using widths at baseline
alternative label: chromatographic peak resolution calculated by the ASTM method
definition: Chromatographic peak resolution that is calculated by the USP <621> method using peak widths measured at the baseline. [Allotrope]

chromatographic peak resolution using peak width at half-height
type: Class
subclass of: chromatographic peak resolution
alternative label: chromatographic peak resolution by USP using widths at half-height
alternative label: chromatographic peak resolution calculated by EP
alternative label: chromatographic peak resolution calculated by JP-27
definition: Chromatographic peak resolution that is calculated using peak widths measured at the half height. [Allotrope]

chromatographic peak resolution using statistical moments
type: Class
subclass of: chromatographic peak resolution
definition: Chromatographic peak resolution that is calculated based on peak widths derived from the second statistical moment (peak variance). [Allotrope]

capacity factor (chromatography)
type: Class
subclass of: chromatography peak facet
license: iupac-license
rights: iupac-copyright
alternative label: capacity ratio
alternative label: mass distribution ratio
alternative label: partition ratio
alternative label: retention factor
definition: The capacity factor is a chromatography peak facet that is a measure of the time the sample component resides in the stationary phase relative to the time it resides in the mobile phase: it expresses how much longer a sample component is retarded by the stationary phase than it would take to travel through the column with the velocity of the mobile phase. [IUPAC]

peak selectivity (chromatography)
type: Class
subclass of: chromatography peak facet
alternative label: peak selectivity
definition: A chromatography peak facet that denotes the ratio of the peak's capacity factor to that of another specified peak. [Allotrope]

chromatographic peak asymmetry factor
type: Class
subclass of: Class
  intersection of: AFR_0001088
  intersection of: chromatography peak facet
alternative label: tailing factor
change note: 2022-012-07 Changed definition. [Allotrope]
definition: A chromatographic peak asymmetry factor is a chromatography peak facet that is equivalent to the ratio of the time or volume from the center point of a peak to its trailing edge to that of its fronting edge at a specified peak height. [Allotrope]

asymmetry factor measured at 5 % height
type: Class
subclass of: chromatographic peak asymmetry factor
alternative label: asymmetry factor by EP
alternative label: asymmetry factor by JP-27
alternative label: asymmetry factor measured at 5 percent height
alternative label: asymmetry factor measured at 5% height
alternative label: tailing factor by USP <621> method
definition: A quantitative asymmetry factor using 5% peak height. [Allotrope]

statistical skew (chromatography)
type: Class
subclass of: chromatographic peak asymmetry factor
alternative label: statistical skew
definition: A quantitative asymmetry factor using the third statistical moment calculated from the peak. [Allotrope]

number of theoretical plates (chromatography)
type: Class
subclass of: chromatography peak facet
alternative label: number of theoretical plates
definition: A chromatography peak facet that measures the amount of band broadening that occurs during the separation and thus the efficiency of the chromatographic system. [Allotrope]

number of theoretical plates by tangent method
type: Class
subclass of: number of theoretical plates (chromatography)
alternative label: number of theoretical plates by ASTM
alternative label: number of theoretical plates by USP method
definition: The number of theoretical plates calculated based on the peak width measured at the baseline. [Allotrope]

number of theoretical plates by peak width at half height
type: Class
subclass of: number of theoretical plates (chromatography)
alternative label: number of theoretical plates by EP method
alternative label: number of theoretical plates by JP method
definition: The number of theoretical plates calculated based on the peak width measured at half height. [Allotrope]

autosampler setting (chromatography)
type: Class
subclass of: Class
  intersection of: chromatography system setting
  intersection of: Restriction
    on property: AFX_0002704
    some values from: Class
      intersection of: AFQ_0000036
      intersection of: Restriction
        on property: RO_0000080
        some values from: AFE_0001711
alternative label: autosampler setting
change note: 2019-07-01 Changed specification target to be a device configuration. [Allotrope]
definition: A parameter specification specific to the sampler device in the chromatography system. [Allotrope]

autosampler injection volume setting (chromatography)
type: Class
subclass of: autosampler setting (chromatography)
subclass of: AFR_0001577
alternative label: autosampler injection volume setting
alternative label: injection volume
alternative label: injection volume setting
definition: An autosampler parameter specification that specifies the volume of sample the autosampler is to inject into the mobile phase for separation. [Allotrope]

pump setting (chromatography)
type: Class
subclass of: Class
  intersection of: chromatography system setting
  intersection of: Restriction
    on property: AFX_0002704
    some values from: Class
      intersection of: AFQ_0000036
      intersection of: Restriction
        on property: RO_0000080
        some values from: AFE_0000499
alternative label: pump parameter setting
change note: 2019-07-01 Changed specification target to be a device configuration. [Allotrope]
definition: A parameter specification specific to the pump device in the chromatography system. [Allotrope]

pump mobile phase ratio setting (chromatography)
type: Class
subclass of: AFR_0001659
subclass of: Class
  intersection of: AFR_0001243
  intersection of: pump setting (chromatography)
change note: 2019-07-01 Changed specification target to be a device configuration. [Allotrope]
definition: A pump parameter specification that specifies the mobile phase ratio in the eluent. [Allotrope]

pump flow rate setting (chromatography)
type: Class
subclass of: AFR_0001660
subclass of: Class
  intersection of: AFR_0001243
  intersection of: pump setting (chromatography)
change note: 2019-07-01 Changed specification target to be a device configuration. [Allotrope]
definition: A pump parameter specification that specifies the total flow rate of the combined mobile phases (eluent). [Allotrope]

washing repetition setting
type: Class
subclass of: autosampler setting (chromatography)
subclass of: AFR_0001243
subclass of: AFR_0001678
change note: 2020-01-06 Moved to chromatography domain. [Allotrope]
change note: 2020-05-11 Fixed definition. [Allotrope]
definition: Washing repetition setting is a setting that sets the number of repetitions of the washing. [Allotrope]

washing duration setting
type: Class
subclass of: autosampler setting (chromatography)
subclass of: AFR_0001243
subclass of: AFR_0001815
change note: 2020-01-06 Moved to chromatography domain. [Allotrope]
definition: Washing duration setting is a setting that sets the duration of the washing. [Allotrope]

washing vial position setting
type: Class
subclass of: autosampler setting (chromatography)
subclass of: AFR_0001243
subclass of: AFR_0001973
change note: 2020-01-06 Moved to chromatography domain. [Allotrope]
definition: Washing vial position setting is a setting that sets the position of the washing vial. [Allotrope]

washing solvent type
type: Class
subclass of: autosampler setting (chromatography)
subclass of: AFR_0001243
subclass of: AFR_0001277
change note: 2020-01-06 Moved to chromatography domain. [Allotrope]
definition: Washing solvent type is a setting that sets the type of washing solvent to use. [Allotrope]

autosampler temperature setting
type: Class
subclass of: autosampler setting (chromatography)
subclass of: AFR_0001243
subclass of: AFR_0001255
change note: 2020-01-06 Moved to chromatography domain. [Allotrope]
definition: An autosampler temperature setting is a setting that sets the temperature within the autosampler. [Allotrope]

maximum flow rate change setting
type: Class
subclass of: AFR_0001243
alternative label: maximum flow gradient
alternative label: pump maximum flow rate change setting
definition: Maximum flow rate change setting is a control setting that sets the maximum rate at which the pump is allowed to change the % of solvents being flowed. [Allotrope]

chromatography system setting
type: Class
subclass of: AFR_0001505
definition: A parameter specification specific to a chromatography system. [Allotrope]

autosampler injection trigger enable setting
type: Class
subclass of: autosampler setting (chromatography)
subclass of: AFR_0002280
alternative label: analysis enable
alternative label: cooler with range enable
definition: Autosampler injection trigger enable setting is a setting that activates or deactivates triggering of injection based on a specified temperature range. [Allotrope]
example: Cooler (with range) can be enabled in order to trigger injection once autosampler temperature has reached a certain value. Enabling the setting also involves activation of the cooler.

autosampler injection trigger temperature setting
type: Class
subclass of: autosampler setting (chromatography)
definition: Autosampler injection trigger temperature setting is a setting that specifies the temperature range for triggering injection. [Allotrope]

mobile phase delivery duration setting
type: Class
subclass of: chromatography system setting
subclass of: Restriction
  on property: AFX_0000553
  some values from: mobile phase delivery
subclass of: Restriction
  on property: AFX_0002704
  some values from: AFR_0000966
definition: Mobile phase delivery duration setting is a chromatography system setting that sets the total duration of mobile phase delivery. [Allotrope]

frit size
type: Class
subclass of: AFR_0001286
subclass of: Restriction
  on property: AFX_0002704
  some values from: AFQ_0000244
definition: Frit size is a column specification that indicates the nominal size of pores in a frit designed to exclude particles larger than the specified size. [Allotrope]

baseline at start of integration setting (chromatography)
type: Class
subclass of: AFR_0001644
subclass of: AFR_0001696
alternative label: baseline at start of integration setting
alternative label: baseline now
alternative label: force baseline
definition: Baseline at start of integration setting is a data processing setting that sets the ordinate value at the start of the integration baseline to the the value of the signal at the time indicated. [Allotrope]

peak end setting (chromatography)
type: Class
subclass of: AFR_0001644
subclass of: AFR_0001696
alternative label: force split peak
alternative label: peak end setting
alternative label: split peak
definition: Peak end setting is a data processing setting that sets the peak end to the value of the abscissa at the time indicated. [Allotrope]

baseline boundary setting (chromatography)
type: Class
subclass of: AFR_0001644
subclass of: AFR_0001696
alternative label: baseline boundary setting
alternative label: force valley baselines
definition: Baseline boundary setting is a data processing setting that sets the ordinate values at the boundaries of the baseline of integration to the actual signal values at the peak start and peak end. [Allotrope]

integration with peak detection enabled setting (chromatography)
type: Class
subclass of: AFR_0001685
subclass of: AFR_0001696
subclass of: AFR_0002280
alternative label: enable integration
alternative label: integration on/off
alternative label: integration with peak detection enabled setting
change note: 2020-12-04 Fixed flag parent. [Allotrope]
definition: Integration with peak detection enabled setting is a data processing setting that allows the integration process to detect peaks for the time period specified. [Allotrope]

negative peak detection enabled setting (chromatography)
type: Class
subclass of: AFR_0001685
subclass of: AFR_0001696
subclass of: AFR_0002280
alternative label: enable negative peak detection
alternative label: negative peak detection enabled setting
alternative label: negative peaks on/off
change note: 2020-12-04 Fixed flag parent. [Allotrope]
definition: Negative peak detection enabled is a data processing setting that allows peaks to be detected below the baseline for the time specified. [Allotrope]

area threshold for peak integration setting (chromatography)
type: Class
subclass of: AFR_0001696
alternative label: area reject
alternative label: area threshold for peak integration setting
alternative label: set minimum area
definition: Area threshold for peak integration setting is a data processing setting that sets a minimum or maximum area for a peak to be included in the integrated peak list. [Allotrope]

height threshold for peak integration setting (chromatography)
type: Class
subclass of: AFR_0001696
alternative label: height reject
alternative label: height threshold for peak integration setting
alternative label: set minimum height
definition: Height threshold for peak integration setting is a data processing setting that sets a minimum or maximum height for a peak to be included in the integrated peak list. [Allotrope]

expected average peak width setting (chromatography)
type: Class
subclass of: AFR_0001696
alternative label: expected average peak width setting
alternative label: peak width
alternative label: set peak width
definition: Expected average peak width setting is a data processing setting that indicates a typical peak width for the time period specified. [Allotrope]

shoulder detection enabled setting (chromatography)
type: Class
subclass of: AFR_0001685
subclass of: AFR_0001696
subclass of: AFR_0002280
alternative label: detect shoulders on/off
alternative label: enable shoulder detection
alternative label: shoulder detection enabled setting
change note: 2020-12-04 Fixed flag parent. [Allotrope]
definition: Shoulder detection enabled setting is a data processing setting that allows the integration process to interpolate a baseline for the smaller peak of overlapping peaks for the time period specified. [Allotrope]

signal-to-noise ratio (USP621)
type: Class
subclass of: AFR_0001197
alternative label: signal-to-noise (USP621)
definition: Signal-to-noise ratio (USP621) is a signal-to-noise ratio (peak-to-peak) where the noise is calculated over a period that is a minimum of 5 times the peak width. [Allotrope]

signal-to-noise ratio (EP2.2.46)
type: Class
subclass of: AFR_0001197
alternative label: signal-to-noise (EP2.2.46)
alternative label: signal-to-noise (JP-G20)
alternative label: signal-to-noise ratio (JP-G20)
definition: Signal-to-noise ratio (EP2.2.46) is a signal-to-noise ratio (peak-to-peak) where the noise is calculated over a period that is a minimum of 20 times the peak width. [Allotrope]

retention time window lower offset
type: Class
subclass of: chromatography peak facet
definition: The retention time window lower offset is the extent of the retention time window in time units below the target retention time. [PSI/MS]
scope note: The lower and upper offsets may be asymmetric about the target time. [PSI/MS]

retention time window upper offset
type: Class
subclass of: chromatography peak facet
definition: The retention time window upper offset is the extent of the retention time window in time units above the target retention time. [PSI/MS]
scope note: The lower and upper offsets may be asymmetric about the target time. [PSI/MS]

retention time window width
type: Class
subclass of: chromatography peak facet
definition: The retention time window width it the full width of a retention time window for a chromatographic peak. [PSI/MS]

chromatography data system method
type: Class
subclass of: AFR_0001501
subclass of: Restriction
  on property: AFX_0002704
  some values from: Class
    intersection of: BFO_0000015
    intersection of: Restriction
      on property: RO_0000057
      some values from: chromatography data system
alternative label: CDS method
alternative label: CDS project
alternative label: chromatography data system project
definition: A chromatography data system method is a plan specification that specifies how a process is to be executed on a chromatography data system. [Allotrope]

column brand name
type: Class
subclass of: AFR_0001680
definition: A column brand name is a brand name of a chromatography column that can be based on the brand name of the substrate and its chemistry modification. [Allotrope]

chromatography column chemistry type
type: Class
subclass of: AFR_0001277
alternative label: chromatography column chemistry classification
alternative label: chromatography column chemistry classification (datum)
alternative label: chromatography column chemistry type (datum)
definition: A chromatography column chemistry type is a classification datum that classifies a chromatography column by the stationary phase and/or chromatographic packing used. [Allotrope]

chromatography column length
type: Class
subclass of: AFR_0001817
subclass of: Restriction
  on property: AFX_0002816
  some values from: chromatography column length (quality)
alternative label: chromatography column length (datum)
definition: A chromatography column length (datum) is a length datum that quantifies the length of a chromatography column. [Allotrope]

column inner diameter
type: Class
subclass of: AFR_0002108
subclass of: Restriction
  on property: AFX_0002816
  some values from: column inner diameter (quality)
alternative label: column inner diameter (datum)
definition: A column inner diameter (datum) is an inner diameter quantifies the inner diameter of a column. [Allotrope]

system flow rate profile
type: Class
subclass of: AFR_0002051
definition: A system flow rate profile is a flow rate profile of the flow rate through the system pump of the chromatograph. [Allotrope]

sample flow rate profile
type: Class
subclass of: AFR_0002051
definition: A sample flow rate profile is a flow rate profile of the flow rate through the sample pump of the chromatograph. [Allotrope]

asymmetry factor measured at 10 % height
type: Class
subclass of: chromatographic peak asymmetry factor
alternative label: asymmetry factor measured at 10 percent of height
alternative label: asymmetry factor measured at 10% height
change note: 2022-11-28 Added alt label. [Allotrope]
change note: 2022-11-28 Changed pref label and definition. [Allotrope]
definition: A quantitative asymmetry factor calculated at 10 percent of its peak height. [Allotrope]

height equivalent to a theoretical plate
type: Class
subclass of: chromatography peak facet
alternative label: HETP
definition: A height equivalent to a theoretical plate is a chromatography peak facet quantifying the ratio of the absorption bed length divided by the number of theoretical plates in the absorption bed. [Wikipedia]

density of theoretical plates (chromatography)
type: Class
subclass of: chromatography peak facet
alternative label: plate density
definition: A density of theoretical plates (chromatography) is a chromatography peak facet that measures the amount of band broadening normalized per length unit that occurs during the separation and thus the efficiency of the chromatographic system. [Allotrope]

retention volume
type: Class
subclass of: AFR_0001101
subclass of: chromatography peak facet
subclass of: Restriction
  on property: AFX_0002816
  some values from: retention volume (quality)
definition: Retention volume is a chromatography peak facet that indicates the acquisition volume at the maximum detector response attributed to the peak (analyte band). [Allotrope]

acquisition volume
type: Class
subclass of: AFR_0001843
subclass of: Restriction
  on property: IAO_0000136
  some values from: AFP_0003095
definition: The acquisition volume is the volume elapsed through the chromatography column between the injection of the sample and when the signal is captured. [Allotrope]

chromatogram data cube
type: Class
subclass of: AFR_0000992
subclass of: DataSet
subclass of: Restriction
  on property:
    inverse of: AFX_0002737
  some values from: AFR_0000236
definition: A chromatogram data cube is a data cube that represents a chromatogram. [Allotrope]

chromatography column particle size
type: Class
subclass of: AFR_0002027
alternative label: chromatography column particle size (datum)
definition: A chromatography column particle size is a particle size datum that quantifies the dimensions of packing particles in a chromatography column. [Allotrope]

chromatography column document
type: Class
subclass of: IAO_0000310
subclass of: Restriction
  on property: IAO_0000136
  some values from: AFE_0000217
definition: A chromatography column document is a document that encompasses the information associated with a chromatography column. [Allotrope]

chromatography column fill type
type: Class
subclass of: AFR_0001277
definition: A chromatography column fill type is a classification datum that classifies a chromatography column by the form of the stationary phase in the column. [Allotrope]
example: Packed, WCOT, PLOT, or SCOT

chromatography column film thickness
type: Class
subclass of: AFR_0002432
alternative label: chromatography column film thickness (datum)
definition: A chromatography column film thickness is a thickness datum that quantifies the thickness of the film coating in a chromatography column. [Allotrope]

sample flow rate data cube
type: Class
subclass of: AFR_0000992
subclass of: DataSet
subclass of: Restriction
  on property:
    inverse of: AFX_0002737
  some values from: sample flow rate profile
definition: A sample flow rate data cube is a data cube that represents the flow rate through the sample pump of a chromatograph. [Allotrope]

system flow rate data cube
type: Class
subclass of: AFR_0000992
subclass of: DataSet
subclass of: Restriction
  on property:
    inverse of: AFX_0002737
  some values from: system flow rate profile
definition: A system flow rate data cube is a data cube that represents the flow rate through the system pump of a chromatograph. [Allotrope]

gradient segment aggregate document
type: Class
subclass of: AFC_0000160
subclass of: IAO_0000310
subclass of: Restriction
  on property: RO_0002351
  some values from: gradient segment document
definition: A gradient segment aggregate document is a document that aggregates gradient segment documents. [Allotrope]

gradient segment document
type: Class
subclass of: IAO_0000310
subclass of: Restriction
  on property: IAO_0000136
  some values from: Class
    intersection of: AFR_0001659
    intersection of: Restriction
      on property: AFX_0002800
      some values from: gradient profile setting (chromatography)
definition: A gradient segment document is a document that encompasses the information associated with a step in some gradient profile. [Allotrope]

gradient profile setting (chromatography)
type: Class
subclass of: AFR_0002415
alternative label: gradient profile setting
definition: A gradient profile setting is a control profile setting that specifies the profile of the ratio of solvents in the mobile phase used over the course of a chromatographic separation. [Allotrope]

hold-up volume
type: Class
subclass of: AFR_0001843
subclass of: Restriction
  on property: AFX_0002816
  some values from: hold-up volume (quality)
definition: A hold-up volume is a quality quantification facet that quantifies the hold-up volume of a chromatographic column. [Allotrope]

peak width at inflection
type: Class
subclass of: AFR_0001075
definition: A peak width at inflection is the peak width calculated by the length between its two inflection points. [Allotrope]

column void volume time
type: Class
subclass of: AFR_0000937
alternative label: column dead time
alternative label: column dwell time
alternative label: column void time
alternative label: dead time
alternative label: dwell time
alternative label: void time
definition: A column void volume time is the time required for an unretained compound to traverse the column and exit. [Allotrope]

column compartment temperature setting
type: Class
subclass of: AFR_0001243
subclass of: AFR_0001255
subclass of: column compartment setting (chromatography)
definition: A column compartment temperature setting is a temperature setting that specifies the temperature of some column compartment. [Allotrope]

asymmetry factor measured at 4.4 % height
type: Class
subclass of: chromatographic peak asymmetry factor
alternative label: asymmetry factor measured at 4.4 percent height
alternative label: asymmetry factor measured at 4.4% height
change note: 2022-11-28 Changed pref label, added alt label. [Allotrope]
definition: A quantitative asymmetry factor equivalent to the ratio of the time or volume from the center point of a peak to its trailing edge to that of its fronting edge at 4.4 % of the peak height. [Allotrope]

asymmetry factor squared measured at 4.4 % height
type: Class
subclass of: chromatographic peak asymmetry factor
alternative label: asymmetry factor squared measured at 4.4 percent height
alternative label: asymmetry factor squared measured at 4.4% height
change note: 2022-11-28 Added alt label. [Allotrope]
definition: A quantitative asymmetry factor equivalent to the square of ratio of the time or volume from the center point of a peak to its trailing edge to that of its fronting edge at 4.4 % of the peak height. [Allotrope]

asymmetry factor squared measured at 10 % height
type: Class
subclass of: chromatographic peak asymmetry factor
alternative label: asymmetry factor squared measured at 10 percent height
alternative label: asymmetry factor squared measured at 10% height
change note: 2022-11-28 Added alt label. [Allotrope]
definition: A quantitative asymmetry factor equivalent to the square of ratio of the time or volume from the center point of a peak to its trailing edge to that of its fronting edge at 10 % of the peak height. [Allotrope]

asymmetry factor measured at baseline
type: Class
subclass of: chromatographic peak asymmetry factor
definition: A quantitative asymmetry factor equivalent to the square of ratio of the time or volume from the center point of a peak to its trailing edge to that of its fronting edge. [Allotrope]

number of theoretical plates measured at 60.7 % of peak height
type: Class
subclass of: number of theoretical plates (chromatography)
alternative label: number of theoretical plates measured at 60.7 percent of peak height
alternative label: number of theoretical plates measured at 60.7% of peak height
change note: 2022-11-28 Added alt label. [Allotrope]
definition: The number of theoretical plates calculated by the peak width at 60.7 % of peak height (2 sigma for a gaussian peak). [Allotrope]

number of theoretical plates measured at 32.4 % of peak height
type: Class
subclass of: number of theoretical plates (chromatography)
alternative label: number of theoretical plates measured at 32.4 percent of peak height
alternative label: number of theoretical plates measured at 32.4% of peak height
change note: 2022-11-28 Added alt label. [Allotrope]
definition: The number of theoretical plates calculated by the peak width at 32.4 % of peak height (3 sigma for a gaussian peak). [Allotrope]

number of theoretical plates measured at 13.4 % of peak height
type: Class
subclass of: number of theoretical plates (chromatography)
alternative label: number of theoretical plates measured at 13.4 percent of peak height
alternative label: number of theoretical plates measured at 13.4% of peak height
change note: 2022-11-28 Added alt label. [Allotrope]
definition: The number of theoretical plates calculated by the peak width at 13.4 % of peak height (4 sigma for a gaussian peak). [Allotrope]

number of theoretical plates measured at 4.4 % of peak height
type: Class
subclass of: number of theoretical plates (chromatography)
alternative label: number of theoretical plates measured at 4.4 percent of peak height
alternative label: number of theoretical plates measured at 4.4% of peak height
change note: 2022-11-28 Added alt label. [Allotrope]
definition: The number of theoretical plates calculated by the peak width at 4.4 % of peak height (5 sigma for a gaussian peak). [Allotrope]

peak leading edge time at 5 % of peak height
type: Class
subclass of: chromatography peak facet
alternative label: peak leading edge time at 5 percent of peak height
alternative label: peak leading edge time at 5% of peak height
change note: 2022-11-28 Added alt label. [Allotrope]
definition: The time or volume from the center point of a peak to its leading edge at 5 % of the peak height. [Allotrope]

expected retention time
type: Class
subclass of: chromatography peak facet
definition: An expected retention time is a peak facet that indicates the expected retention time as specified by the data processing method. [Allotrope]

chromatography peak type
type: Class
subclass of: AFR_0003070
subclass of: Restriction
  on property: AFX_0002806
  some values from: chromatography peak
alternative label: peak type
definition: A chromatography peak type is a classification datum that classifies a chromatography peak by the role played by the substance it is a proxy for in the chromatographic process. [Allotrope]
example: main component, mobile phase, excipient
scope note: It is mainly used to determine if it is relevant to the particular analysis.

chromatography separation type
type: Class
subclass of: AFR_0001277
subclass of: Restriction
  on property: AFX_0002806
  some values from: AFP_0000422
definition: A chromatography separation type is a classification datum that classifies the underlying separation process used in the chromatographic process. [Allotrope]
example: reversed phase, ion exchange, normal phase, ligand

impurity chromatography peak type
type: Class
subclass of: chromatography peak type
alternative label: impurity type
definition: An impurity type is a classification datum that classifies a peak by the impurity role played by the substance it is a proxy for in the chromatographic process. [Allotrope]

peak symmetry factor measured at 5 % peak height
type: Class
subclass of: chromatography peak facet
alternative label: peak symmetry factor measured at 5% peak height
definition: A chromatography peak facet equivalent to the ratio of the time or volume from the center point of a peak to its fronting edge divided by the entire width of the peak at 5% of the peak height. [Allotrope]

number of theoretical plates by peak width at half height (JP14)
type: Class
subclass of: number of theoretical plates (chromatography)
alternative label: number of theoretical plates by JP14 method
definition: The number of theoretical plates calculated based on the peak width measured at half height, adjusted to use 5.55 as the square of the standard deviation in accordance with JP14. [Allotrope]
scope note: Japanese Pharmacopeia previously required use of a different constant value to the one currently used in alignment with European Pharmacopeia for the square of the standard deviation in this method. This term represents results calculated using that alternate constant value.

column compartment setting (chromatography)
type: Class
subclass of: chromatography system setting
subclass of: Class
  intersection of: chromatography system setting
  intersection of: Restriction
    on property: AFX_0002704
    some values from: Class
      intersection of: AFQ_0000036
      intersection of: Restriction
        on property: RO_0000080
        some values from: column compartment
alternative label: column compartment setting
definition: A parameter specification specific to the column compartment in the chromatography system. [Allotrope]

minimum peak detection slope setting (chromatography)
type: Class
subclass of: AFR_0001696
definition: A minimum peak detection slope setting is a data processing setting that defines the minimum slope of the signal (rate of change of the signal per acquisition time) at or above which the start of a peak is detected. [Allotrope]

chromatography column void volume
type: Class
subclass of: AFR_0001843
license: iupac-license
rights: iupac-copyright
alternative label: V0
alternative label: interparticle volume of the column
alternative label: interstitial volume
alternative label: void volume
change note: 2026-06-12 Changed prefLabel. [Allotrope]
definition: A void volume is the volume occupied by the mobile phase between the particles in the packed section of a column. [IUPAC]
note: In gas chromatography, the symbol V_G may be used for the interparticle volume of the column. In the ideal case, neglecting any extra-column volume, V_G is equal to the corrected gas hold-up volume (V0_M). V_G = V0_M = V_M [IUPAC]
note: In liquid chromatography, the interparticle volume is equal to the mobile-phase hold-up volume (V_M) in the ideal case, neglecting any extra-column volume. [IUPAC]

partition coefficient
type: Class
subclass of: AFR_0001583
alternative label: Kav
definition: A partition coefficient is a quantity quantification facet quantifying the gel phase distribution constant in gel filtration. [Allotrope]

normalized plate count
type: Class
subclass of: number of theoretical plates (chromatography)
definition: A normalized plate count is the number of theoretical plates normalized on a per meter basis. [Allotrope]

suitability calculations enabled setting
type: Class
subclass of: AFR_0002805
definition: A suitability calculations enabled setting is a data processing setting that indicates if system suitability results are to be calculated. [Allotrope]

unknown suitability calculations enabled setting
type: Class
subclass of: AFR_0002805
definition: An unknown suitability calculations enabled setting is a data processing setting that indicates if system suitability results are to be calculated for unknowns. [Allotrope]

suitability pharmacopoeia setting
type: Class
subclass of: AFR_0001696
definition: A suitability pharmacopoeia setting is a data processing setting that indicates which pharmacopoeia calculation methods are to be used. [Allotrope]

half height multiplier plate count setting (USP)
type: Class
subclass of: AFR_0001696
definition: A half height multiplier plate count setting (USP) is a data processing setting that indicates at which peak height the USP plate count should be calculated. [Allotrope]

half height multiplier resolution setting (USP)
type: Class
subclass of: AFR_0001696
definition: A half height multiplier resolution setting (USP) is a data processing setting that indicates at which peak height the USP resolution should be calculated. [Allotrope]

blank injection noise calculation setting
type: Class
subclass of: AFR_0002805
definition: A blank injection noise calculation setting is a data processing setting that indicates that a blank injection is to be used for calculating baseline noise. [Allotrope]

half height multiplier signal to noise setting (USP)
type: Class
subclass of: AFR_0001696
definition: A half height multiplier signal to noise setting (USP) is a data processing setting that indicates the half height multiplier that is to be used in determining the baseline region for calculating noise in USP S/N calculations. [Allotrope]

half height multiplier signal to noise setting (EP)
type: Class
subclass of: AFR_0001696
definition: A half height multiplier signal to noise setting (EP) is a data processing setting that indicates the half height multiplier that is to be used in determining the baseline region for calculating noise in EP S/N calculations. [Allotrope]

half height multiplier signal to noise setting (JP)
type: Class
subclass of: AFR_0001696
definition: A half height multiplier signal to noise setting (JP) is a data processing setting that indicates the half height multiplier that is to be used in determining the baseline region for calculating noise in JP S/N calculations. [Allotrope]

mass chromatogram data cube
type: Class
subclass of: chromatogram data cube
subclass of: Restriction
  on property:
    inverse of: AFX_0002737
  some values from: mass chromatogram
definition: A mass chromatogram data cube is a data cube that is a representation of some mass chromatogram. [Allotrope]

chromatographic peak resolution using Foley-Dorsey method
type: Class
subclass of: chromatographic peak resolution
definition: A chromatographic peak resolution that is calculated using peak widths from the Foley Dorsey equation for asymmetric peaks. [Allotrope]

chromatographic peak resolution using peak widths at 4.4 % height
type: Class
subclass of: chromatographic peak resolution
alternative label: chromatographic peak resolution using peak widths at 4.4% height
definition: A chromatographic peak resolution that is calculated using peak widths at 4.4 % peak height. [Allotrope]

chromatography column separation mode
type: Class
subclass of: AFR_0001277
subclass of: Restriction
  on property: AFX_0002806
  some values from: AFE_0000217
definition: A chromatography column separation mode is a classification datum that classifies a column by the type of chromatography it supports. [Allotrope]

stationary phase document
type: Class
subclass of: IAO_0000310
subclass of: Restriction
  on property: IAO_0000136
  some values from: Class
    intersection of: AFM_0000275
    intersection of: Restriction
      on property: RO_0000087
      some values from: stationary phase
definition: A stationary phase document is a document that encapsulates the information associated with a stationary phase. [Allotrope]

substrate document
type: Class
subclass of: IAO_0000310
subclass of: Restriction
  on property: IAO_0000136
  some values from: Class
    intersection of: AFM_0000275
    intersection of: Restriction
      on property: RO_0000087
      some values from: AFRL_0000211
definition: A substrate document is a document that encapsulates the information associated with a substrate. [Allotrope]

substrate permeability type
type: Class
subclass of: AFR_0001277
subclass of: Restriction
  on property: AFX_0002806
  some values from: PATO_0000970
definition: A substrate permeability type is a classification datum that classifies the permeability of a substrate. [Allotrope]

substrate material description
type: Class
subclass of: AFR_0000922
subclass of: Restriction
  on property: IAO_0000136
  some values from: Class
    intersection of: AFM_0000275
    intersection of: Restriction
      on property: RO_0000087
      some values from: AFRL_0000211
definition: A substrate material description is a description of the material used in a substrate. [Allotrope]

phase chemistry type
type: Class
subclass of: AFR_0001277
subclass of: Restriction
  on property: AFX_0002806
  some values from: Class
    intersection of: AFM_0000275
    intersection of: Restriction
      on property: RO_0000087
      some values from: stationary phase
definition: A phase chemistry type is a classification datum that classifies the chemistry of some stationary phase. [Allotrope]

acidity (hydrophobic subtraction)
type: Class
subclass of: AFR_0001583
subclass of: Restriction
  on property: AFX_0002816
  some values from: Class
    intersection of: AFQ_0000367
    intersection of: Restriction
      on property: RO_0000080
      some values from: Class
        intersection of: AFM_0000275
        intersection of: Restriction
definition: An acidity is a quality quantification facet that quantifies the acidity of a chromatographic stationary phase. [Allotrope]

basicity (hydrophobic subtraction)
type: Class
subclass of: AFR_0001583
subclass of: Restriction
  on property: AFX_0002816
  some values from: Class
    intersection of: AFQ_0000368
    intersection of: Restriction
      on property: RO_0000080
      some values from: Class
        intersection of: AFM_0000275
        intersection of: Restriction
definition: A basicity is a quality quantification facet that quantifies the basicity of a chromatographic stationary phase. [Allotrope]

hydrophobicity (hydrophobic subtraction)
type: Class
subclass of: AFR_0001583
subclass of: Restriction
  on property: AFX_0002816
  some values from: Class
    intersection of: PATO_0001884
    intersection of: Restriction
      on property: RO_0000080
      some values from: Class
        intersection of: AFM_0000275
        intersection of: Restriction
definition: A hydrophobicity is a quality quantification facet that quantifies the hydrophobicity of a chromatographic stationary phase. [Allotrope]

steric resistance (hydrophobic subtraction)
type: Class
subclass of: AFR_0001583
subclass of: Restriction
  on property: AFX_0002816
  some values from: Class
    intersection of: AFQ_0000369
    intersection of: Restriction
      on property: RO_0000080
      some values from: Class
        intersection of: AFM_0000275
        intersection of: Restriction
definition: A steric resistance is the quality quantification facet that quantifies the steric resistance of a chromatographic stationary phase. [Allotrope]

polarizability (hydrophobic subtraction)
type: Class
subclass of: AFR_0001583
subclass of: Restriction
  on property: AFX_0002816
  some values from: Class
    intersection of: PATO_0002189
    intersection of: Restriction
      on property: RO_0000080
      some values from: Class
        intersection of: AFM_0000275
        intersection of: Restriction
definition: A polarizability is the quality quantification facet that quantifies the polarizability of a chromatographic stationary phase. [Allotrope]

retention index
type: Class
subclass of: chromatography peak facet
license: iupac-license
rights: iupac-copyright
definition: The retention index of a sample component is a number, obtained by interpolation (usually logarithmic), relating the adjusted retention volume (time) or the retention factor of the sample component to the adjusted retention volumes (times) of two standards eluted before and after the peak of the sample component. [IUPAC]

mobile phase
type: Class
subclass of: chromatographic phase
alternative label: mobile phase role
definition: Mobile phase is the chromatographic phase role of a fluid material entity in a chromatography process to act as the fluid that percolates through or along the stationary bed in chromatography. [Allotrope, CHMO]

stationary phase
type: Class
subclass of: chromatographic phase
definition: A stationary phase is the chromatographic phase role of a solid, gel or liquid material that is the stationary component in chromatography. [Allotrope]

chromatographic phase
type: Class
subclass of: BFO_0000023
definition: One of the portions of material between which the components to be separated are distributed in chromatography. [CHMO]

gaseous mobile phase
type: Class
equivalent class: Class
  intersection of: mobile phase
  intersection of: Restriction
    on property: RO_0000081
    some values from: AFM_0000154
AFX_0002798: true
alternative label: gaseous mobile phase role
definition: Gaseous mobile phase is the mobile phase role of a gaseous material. [Allotrope]

supercritical mobile phase
type: Class
equivalent class: Class
  intersection of: mobile phase
  intersection of: Restriction
    on property: RO_0000081
    some values from: AFM_0000401
AFX_0002798: true
definition: Supercritical mobile phase is the mobile phase role of a supercritical fluid material. [Allotrope]

effluent
type: Class
subclass of: mobile phase
definition: Effluent is a mobile phase eluting from a column. [Allotrope]

eluate
type: Class
subclass of: mobile phase
change note: 2020-12-16 Rephrased definition [Allotrope]
definition: A mobile phase that is analyte material coming from the chromatograph. [Allotrope]

eluent
type: Class
subclass of: mobile phase
definition: Eluent is a mobile phase entering a column to perform a separation of material. [Allotrope]

capillary inner coating role
type: Class
subclass of: AFRL_0000212
alternative label: capillary inner coating
definition: A capillary inner coating role is a coating role of a material that is a coating within a chromatography column. [Allotrope]

endcapping role
type: Class
subclass of: AFRL_0000085
alternative label: endcapping
definition: An endcapping role is a supporting material role with the purpose of preventing chromatographic analytes from interacting with the substrate of the stationary phase. [Allotrope]

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type: Organization

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description: Copyright © 2019 International Union of Pure Applied Chemistry

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description: Reproduced by permission of International Union of Pure and Applied Chemistry